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DNAQF

Sigma-Aldrich

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

Synonyme(s) :

dsDNA quantification

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.84

Niveau de qualité

200

Utilisation

sufficient for 750 assays (2 ml each)

Technique(s)

nucleic acid detection: suitable

Température de stockage

−20°C

Description générale

The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.

Application

DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
  • in neonatal rat ventricular myocytes (NRVMs)
  • in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
  • in mouse embryonic fibroblast cell line (NIH3T3 cells)

Caractéristiques et avantages

  • Specific for the quantitation of nanogram amounts of DNA
  • Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins

Composants de kit seuls

Réf. du produit
Description
  • Detailed technical bulletin 1 ea

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Brian D Fink et al.
PloS one, 7(6), e39430-e39430 (2012-06-30)
It has been suggested that cells that are independent of insulin for glucose uptake, when exposed to high glucose or other nutrient concentrations, manifest enhanced mitochondrial substrate oxidation with consequent enhanced potential and generation of reactive oxygen species (ROS); a
Kausik Kapat et al.
ACS applied materials & interfaces, 9(45), 39235-39248 (2017-10-24)
Cementless fixation for orthopedic implants aims to obviate challenges associated with bone cement, providing long-term stability of bone prostheses after implantation. The application of porous titanium and its alloy-based implants is emerging for load-bearing applications due to their high specific
D Moe et al.
Journal of biochemical and biophysical methods, 28(4), 263-276 (1994-06-01)
The present study was designed to afford a critical review of the effect of proteins on the Hoechst 33258 method for determination of DNA of crude homogenates. A considerable effect of proteins on the fluorescence was observed when the concentration
In vitro cytocompatibility and blood compatibility of polysulfone blend, surface-modified polysulfone and polyacrylonitrile membranes for hemodialysis
Anirban Roy
Royal Society of Chemistry Advances, 5(10), 7023-7034 (2015)
Mohmmad Mardani et al.
Iranian journal of basic medical sciences, 16(6), 763-773 (2013-09-03)
Osteoarthritis is one of the most common diseases in middle-aged population in the world. Cartilage tissue engineering (TE) has been presented as an effort to introduce the best combination of cells, biomaterial scaffolds and stimulating growth factors to produce a
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