D9538
N,N′-(1,2-Dihydroxyethylene)bis-acrylamide
suitable for electrophoresis
Synonyme(s) :
DHEBA, N,N′-Bisacryloyl-1,2-dihydroxy-1,2-ethylenediamine
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A propos de cet article
Formule empirique (notation de Hill) :
C8H12N2O4
Numéro CAS:
Poids moléculaire :
200.19
EC Number:
212-780-1
UNSPSC Code:
12162002
MDL number:
Beilstein/REAXYS Number:
1941875
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electrophoresis: suitable
conductivity
<60 μmho (2% solution)
storage temp.
2-8°C
SMILES string
OC(NC(=O)C=C)C(O)NC(=O)C=C
InChI
1S/C8H12N2O4/c1-3-5(11)9-7(13)8(14)10-6(12)4-2/h3-4,7-8,13-14H,1-2H2,(H,9,11)(H,10,12)
InChI key
ZMLXKXHICXTSDM-UHFFFAOYSA-N
Application
Reversible cross-linker for polyacrylamide gels that produces a gel, which is soluble in dilute (2%) periodic acid at room temperature for 1-2 hr. Gel solubilization is useful when recovering protein from polyacrylamide gels.
Analysis Note
Tested for use as a cross-linking agent for recovery of separated biomolecules.
signalword
Warning
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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U Neumann et al.
Analytical biochemistry, 206(1), 1-5 (1992-10-01)
A simple and rapid method for the quantitation of proteins separated either by sodium dodecyl sulfate-electrophoresis or by isoelectric focusing in slab gels is presented. The method is based on the solubility of polyacrylamide gels crosslinked with N, N'-1, 2-dihydroxyethylenebisacrylamide
A method for the quantitative determination of protein incorporated in solubilized polyacrylamide gels.
J Tas et al.
Analytical biochemistry, 100(2), 264-270 (1979-12-01)
M Galvani et al.
Electrophoresis, 21(17), 3684-3692 (2001-03-29)
A number of cross-linkers that are commonly used in polyacrylamide gels have been incubated with bovine beta-lactoglobulin B and the resulting reaction mixtures were examined by matrix assisted laser desorption/ionization-mass spectrometry. At concentrations of 0.1, 1, and 20 mM of