D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964

buffered aqueous glycerol solution

• Synonyme(s) : Kornberg Polymerase
• Numéro CAS: 9012-90-2
• Numéro de classification (Commission des enzymes): 2.7.7.7 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130924
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.53

Propriétés

• Qualité: for molecular biology
• Forme: buffered aqueous glycerol solution
• Poids mol.: 109 kDa
• Concentration: 5,000-15,000 units/mL
• Numéro d'accès UniProt: P00582
• Activité étrangère: Endonuclease, none detected
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: Escherichia coli K12 ... polA(948356)

Description

Description générale

DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

Application

DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.

Suitable for:

• Highly specific DNA probes by nick translation
• In vitro synthesis of complementary cDNA strand
• In vitro synthesis of DNA
• Produce blunt ends from 5′ and 3′ overhangs

Composants

DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.

Définition de l'unité

One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)