D8281

Anti-Desmin antibody produced in rabbit

whole antiserum

• Synonyme(s) : Anti-Desmin, Desmin Detection Antibody, Rabbit Anti-Desmin
• Numéro MDL: MFCD00162258
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: whole antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Contient: 15 mM sodium azide
• Espèces réactives: chicken, bovine, human, mouse
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Technique(s): indirect immunofluorescence: 1:20 using cultured cells; indirect immunofluorescence: 1:20 using human skeletal muscle sections; western blot: 1:100 using animal or human skeletal muscle.
• Numéro d'accès UniProt: P17661
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DES(1674) ; mouse ... Des(13346)

Description

Description générale

Desmin is an intermediate filament protein found mainly in skeletal, cardiac, and smooth muscle cells. It acts as a marker for tumor origin. It also facilitates mechanical support for muscles, mitochondrial localization, myofibrillogenesis, gene expression regulation, and intracellular signalling. Anti-desmin antibody can be used for immunocytochemical localization of intermediate filaments in all types of muscle cells. It can also be used to stain a wide desmin band of 50,000 to 55,000 molecular weight using immunoblotting assays. Rabbit anti-desmin antibody reacts specifically with desmin in bovine, mouse, chicken and human.

Desmin is mapped to human chromosome 2q35. The desmin protein comprises of a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. In smooth and striated muscles, desmin is localized in the periphery of Z-disk.

Immunogène

desmin from chicken gizzard

Application

Anti-Desmin antibody produced in rabbit has been used in:

• immunohistochemistry of zebrafish intestines sections
• immunodetection in fish embryo samples
• immunolabeling of myofibers

Anti-desmin antibody can be used in immunocytochemistry and western immunoblotting. It can also be used in indirect immunofluorescence.

Actions biochimiques/physiologiques

Desmin plays a crucial role in providing mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Missense mutations in desmin gene leads to hypertrophic cardiomyopathy and systemic myopathy. Mutations in desmin gene impairs the stability of intermediary filaments in cardiomyocytes, contributing to pathophysiology of cardiac diseases.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable