Le milieu de Eagle modifié par Dulbecco (DMEM) est une variante du milieu de Eagle de base (BME) qui contient quatre fois plus d'acides aminés et de vitamines. La formulation d'origine contenait 1 000 mg/l de glucose et servait à la culture de cellules embryonnaires de souris. Depuis, le milieu a été modifié à plusieurs reprises pour convenir aux primocultures de cellules de souris et de poulet ainsi que de diverses cellules normales ou transformées. Chacun de ces milieux apporte une combinaison différente de L-glutamine et de pyruvate de sodium. En outre, le taux de glucose a été augmenté à 4 500 mg/l, d'où le nom "DMEM/High" pour DMEM riche en glucose.
Quantité
Formulated to contain 17.3 grams of powder per liter of medium.
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers
Journal of cellular and molecular medicine, 22(10), 4840-4855 (2018-07-20)
BMSCs are important in replacement therapy of diabetic nephropathy (DN). MiR-124a exerts effect on the differentiation capability of pancreatic progenitor cells. The objective of this study was to explore the molecular mechanisms, the functions of miR-124a and bone marrow mesenchymal
Physapubescin B, a steroidal compound extracted from the plant Physalis pubescens L. (Solanaceae), has been reported to possess anti-cancer potential, whereas the molecular mechanism remains elusive. In this study, we first demonstrated that physapubescin B induced autophagy in human cancer
The American journal of Chinese medicine, 46(3), 673-687 (2018-04-05)
Curcumin is a hydrophobic polyphenol derived from turmeric: the rhizome of the herb Curcumalonga. Autophagy is an evolutionarily conserved process, in which cellular proteins and organelles are engulfed in autophagosome and then fuses with lysosome for degradation. Our previous study
The Journal of biological chemistry, 292(27), 11561-11571 (2017-05-17)
CO2 is a physiological gas normally produced in the body during aerobic respiration. Hypercapnia (elevated blood pCO2 >≈50 mm Hg) is a feature of several lung pathologies, e.g. chronic obstructive pulmonary disease. Hypercapnia is associated with increased susceptibility to bacterial
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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