Accéder au contenu
Merck

Principaux documents

Voir toute la documentation

Passer à

D0428

1 kb DNA Ladder

for DNA electrophoresis

Synonyme(s) :

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue
À vous/RéférenceDisponibilitéPrix
1 vial

Disponible dès aujourd'huiDeSt QuentinFallavier

466,00 €

A propos de cet article

NACRES:
NA.25
UNSPSC Code:
41105335

466,00 €

Disponible dès aujourd'huiDétails

​
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Quality Segment

100, 200

form

liquid

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR™ Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.[1]

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries

Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Questions

1–2 sur 2 questions  

  1. Does this product have biological origin? Or is the DNA synthetically formulated?

    1 réponse

    1. This product has historically been synthetically produced. However, it is best to refer to the lot-specific Certificate of Origin, as the method of manufacture and origin of future lots may be subject to change. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab or click on the link below to request a lot-specific Certificate of Origin:
      https://www.sigmaaldrich.com/product/sigma/d0428#product-documentation

      Utile ?

  2. how to use this product. in what ratio we have to add dye in this.

    1 réponse

    1. This product has been tested successfully at 2 µL, 5µL, and 7 µL., however for general use, a starting volume of 5 µL is recommended. Dilute 5 µL volume of D0428 with 25 µL of gel loading buffer. Product G2526 is used for testing purposes. For best results, a 0.75% gel is recommended.
      See the link below to Gel Loading Buffer, G2526:
      https://www.sigmaaldrich.com/product/sigma/g2526

      Utile ?

Évaluations

Aucune valeur de notation

Filtres actifs