CS0004

Nucléase, micrococcale from Staphylococcus aureus

With reaction buffer and BSA solution

• Synonyme(s) : Endonucléase micrococcale, Endonucléase, micrococcale, MNase
• Numéro CAS: 9013-53-0
• UNSPSC Code: 12352202
• NACRES: NA.77
• Numéro CE : 3.1.31.1 (BRENDA, IUBMB)
• MDL number: MFCD00131731
• Biological source: Staphylococcus aureus

Propriétés

• biological source: Staphylococcus aureus
• form: liquid
• input: bacteria and yeast sample(s)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Application

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Biochem/physiol Actions

Hydrolyse les liaisons 5′-phosphodiester de l'ADN

Other Notes

Remarque : 1 μmol = environ 85 unités A₂₆₀, où une unité A₂₆₀ équivaut à une ΔA₂₆₀ de 1,0 en 30 minutes, à pH 8,8 et à 37 °C, dans un volume réactionnel de 3,55 ml. Trajet optique = 1 cm.

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 3