CHROP
Imprint® Ultra Chromatin Optimization Kit
Kit designed to optimize sonication parameters for ChIP experiments
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| Taille/Référence | Disponibilité | Prix |
|---|---|---|
15 reactions | Vérifier la disponibilité des articles du panier | 333,00 € |
A propos de cet article
NACRES:
NA.54
UNSPSC Code:
41116158
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General description
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
Other Notes
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Cet article | |||
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| description Kit designed to optimize sonication parameters for ChIP experiments | description Complete ChIP reaction in 6 hours in flexible strip well format | description For bisulfite DNA conversion & purification | description - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
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Réf. du produit
Description
FDS & Tarif
Mot-clé
Danger
Codes de danger
Organes cibles
Central nervous system
Classe de stockage
5.1A - Strongly oxidizing hazardous materials
Point d'éclair (°F)
100.4 °F
Point d'éclair (°C)
38 °C
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3
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