AFRC IAH-CC62

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• Conditionnement: tube of 5 μg 99011334-DNA-5UG; pkg of vial of cells 99011334-1VL
• ImmunoDon: BALB/c mouse spleen
• Myélome: NS-1
• Réactivité: Anti-CD26 (formerly WC10), bovine
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgG2b
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse x Mouse hybridoma

Description de la lignée cellulaire

The hybridoma IAH-CC62 secretes antibodies reactive with bovine CD26 - this was formerly known as WC10. This non-lineage specific antigen is present on bovine leukocytes and has a MW of 115 kDa and 39kDa. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentractions appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.

Immunogène

Bovine thymocytes

Application

Immunohistology, Flow cytometry, Immunoprecipation

Milieu de culture

RPMI 1640 + 2 mM glutamine + HT supplement + 10% FBS

Procédure de repiquage

Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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