LB182

98062317, human blood, Lymphoblastoid

• Code UNSPSC : 41106514

Propriétés

• product name: LB182, 98062317
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblastoid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lymphoblastoid, normal, EBV-transformed

Description de la lignée cellulaire

Derived from mononuclear cells from peripheral blood of a 51 year old normal male. Cells were subsequently transformed with EBV. Fibroblasts designated 149BR (Sigma Catalogue number. 90011807) have been isolated from the same individual . This has been confirmed by STR profiling at ECACC. T cells from the same sample have been used for cellular radiosensitivity studies and cell survival analysis after UV-irradiation.

Application

Mutation studies, Toxicity analysis

Milieu de culture

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Procédure de repiquage

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Autres remarques

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