C2015

950220149

• Code UNSPSC : 12352200

Propriétés

• Source biologique: human blood
• Numéro de panel HRC: Not specified
• Mode de croissance: Suspension
• Caryotype: Not Specified
• Morphologie: Lymphoblastoid

Description

Origine de la lignée cellulaire

UK Caucasian

Description de la lignée cellulaire

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

Application

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

Milieu de culture

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Procédure de repiquage

Maintain cultures between 3 x 10⁵ - 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.

Autres remarques

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.