RC-4B/C1

94020810, rat brain, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• product name: RC-4B/C1, 94020810
• Source biologique: rat brain
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial-like
• Produits: Gonadatrophs
• Récepteurs: Gonadotrophin-releasing hormone receptors
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat pituitary adenoma

Description de la lignée cellulaire

RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.

Application

Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.

Milieu de culture

DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 10% CO₂; 34°C. Saturation density is reached at 1x 100,000 cells/cm². Cells may attach lightly and so handling should be kept to a minimum. Care shoul

Autres remarques

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