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TF1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93022307, human blood, Not specified

Synonyme(s) :

Human TF1 Cells, TF1 Cell Culture

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About This Item

Code UNSPSC :
41106514
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

human blood

Conditionnement

tube of 5 μg 93022307-DNA-5UG
pkg of vial of cells 93022307-1VL

Mode de croissance

Suspension

Caryotype

Carries homogeneous chromosomal abnormality (54,X)

Morphologie

Not specified

Produits

Not specified

Récepteurs

Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

Technique(s)

cell culture | mammalian: suitable

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−196°C

Origine de la lignée cellulaire

Human erythroleukaemia

Description de la lignée cellulaire

TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.

Application

TF1 cell line has been used-
  • for tumorigenic studies
  • to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells[1]
  • in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061[2]
  • to study the induction of STAT5 phosphorylation by IL-3[3]

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.

Procédure de repiquage

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

Autres remarques

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Questions

1–7 sur 7 questions  

  1. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 réponse

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  2. How many Cell Line cells are in the vial?

    1 réponse

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  3. Are the Cell Line cells tested for viral pathogens?

    1 réponse

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  4. What medium should I use when I thaw the Cell Line cells?

    1 réponse

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  5. How do I handle Cell Line frozen cells upon arrival?

    1 réponse

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  6. Are my Cell Line cells mycoplasma tested?

    1 réponse

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  7. What passage are my Cell Line cells?

    1 réponse

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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