OX-49

Reactivity: Anti-CD44, rat, 91062501

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myélome: NS0
• Réactivité: Anti-CD44, rat
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgG2a
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse NS0 x Mouse BALB/c spleen

Description de la lignée cellulaire

The antibody detects the CD44 antigen of molecular weight 90kDa found on activated lymphocytes. The antibody does not react with a subset of B lymphocytes.

Immunogène

Rat T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells

Application

FACS, RIA

Milieu de culture

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.