1BR.3.GN

90020509, human skin, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: 1BR.3.GN, 90020509
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human skin fibroblast, transformed

Description de la lignée cellulaire

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmids pSV3gpt and pSV3neo. The cells were selected for pSV2neo only.The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.

Application

DNA repair and cell growth studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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