THM 7.3.5

Reactivity: Anti-NP (4-hydroxy-3 nitrophenacetyl), 87120201

• Code UNSPSC : 12352200

Propriétés

• Source biologique: Mouse + Human Transfectoma
• ImmunoDon: Not applicable
• Myélome: J558L
• Réactivité: Anti-NP (4-hydroxy-3 nitrophenacetyl)
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgM
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse J558L + Mouse VH gene + Human C(μ) gene

Description de la lignée cellulaire

This human chimeric antibody is specifically directed against the hapten-4-hydroxy-3-nitrophenacetyl. V lambda of the endogenous light chain of plasmacytoma line complements VNP of transfected heavy chain to yield specific antibody. Gene marker: gpt.

Milieu de culture

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.