From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.
Application
Human MG-63 cell line has been used:
to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells[1]
to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells[2]
as a positive control for immunostaining procedure[3]
to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells[4]
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Autres remarques
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