HeB5

11033104, mouse liver, Epithelial

• Synonyme(s) : Hepa-1c1c7
• Code UNSPSC : 41106514

Propriétés

• product name: HeB5, 11033104
• Source biologique: mouse liver
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

Hepa-1c1c7, genetic modification, Xenobiotic-Responsive Element (XRE)

Description de la lignée cellulaire

HeB5 is a derivative of Hepa-1c1c7 (Sigma product number 95090613) cell line stably transfected with a firefly luciferase reporter construct bearing Xenobiotic-Responsive Element (XRE) driven by a TATA promoter. HeB5 can be used for detection of mouse CYP1A induction by chemicals.

Milieu de culture

Alpha-MEM + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16μg/ml Blasticidin S HCL.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.