Sp2/0-Ag14-TurboDoma

3092501, mouse unknown/unspecified, Lymphoblast

• Code UNSPSC : 41106514

Propriétés

• product name: Sp2/0-Ag14-TurboDoma, 03092501
• Source biologique: mouse unknown/unspecified
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse x Mouse hybridoma, non-producing, serum-free

Description de la lignée cellulaire

Sp2/0-Ag14 cell line (Sigma Catalogue number. 85072401) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma (Cell Culture Technologies). Suitable for use as a fusion parent for serum-free generation of hybridomas as well as a host for the production of recombinant proteins.

Application

Fusion partner for generation of serum-free hybridomas, host for the expression of recombinant genes.

Milieu de culture

TurboDoma^™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

Procédure de repiquage

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C..

Autres remarques

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Informations légales

TurboDoma is a trademark of Cell Culture Technologies