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Merck
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Principaux documents

CA200

Sigma-Aldrich

cAMP Enzyme Immunoassay Kit, Direct

Synonyme(s) :

cAMP detection kit

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1 KIT
1 240,00 €

1 240,00 €


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1 KIT
1 240,00 €

About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

1 240,00 €


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Technique(s)

ELISA: suitable

Niveau de qualité

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.[1]
Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.

Application

Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium[2] virulent and attenuated macrophages.[3] It has also been used to quantify cAMP synthesis in olfactory epithelium.[4]

Actions biochimiques/physiologiques

Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.[1]

Autres remarques

Allow reagents to warm at room temperature for 30 minutes prior to opening.

Composants de kit seuls

Réf. du produit
Description

  • 0.1 M Hydrochloric Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Direct cAMP Assay Layout Sheet 1 x 1

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • Neutralizing Reagent 1 x 6

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

Afficher tout (14)

Pictogrammes

FlameSkull and crossbonesCorrosion

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

Organes cibles

Respiratory system

Risques supp

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

120.2 °F

Point d'éclair (°C)

49 °C


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Haemodynamic and extracellular matrix cues regulate the mechanical phenotype and stiffness of aortic endothelial cells.
Collins C, Osborne LD, Guilluy C, et al.
Nature Communications, 5, 3984-3984 (2014)
Sujoy Das et al.
PloS one, 5(8), e12039-e12039 (2010-08-14)
Several studies have been reported on the occurrence of sperm motility inhibiting factors in the male reproductive fluids of different mammalian species, but these proteins have not been adequately purified and characterized. A novel sperm motility inhibiting factor (MIF-II) has
Anna Maria D'Ursi et al.
Molecular pharmacology, 69(3), 727-736 (2005-12-08)
Cell-penetrating peptides are able to transport covalently attached cargoes such as peptide or polypeptide fragments of endogenous proteins across cell membranes. Taking advantage of the cell-penetrating properties of the 16-residue fragment penetratin, we synthesized a chimeric peptide that possesses an
Mojtaba Panjehpour et al.
Oncology research, 16(12), 575-585 (2008-03-21)
Adenosine modulates the proliferation, survival, and apoptosis of many different cell types. The present study was performed to investigate the role of adenosine receptors in the human breast cancer cell lines MCF-7 and MDA-MB468. The biological effects of adenosine on
Yang Hu et al.
PloS one, 13(4), e0195553-e0195553 (2018-04-14)
Sensing the environmental signals, the canonical Gα-cAMP/PKA pathway modulates mycelial growth and development, and negatively regulates some secondary metabolism in filamentous fungi, e.g. aflatoxin in Aspergillus nidulans. Here we report the characterization of this signaling pathway in Chaetomium globosum, a

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