Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

C8058

Sigma-Aldrich

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

Synonyme(s) :

Chondroitin sulfate B lyase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

50 UNITS
747,00 €

747,00 €

Date d'expédition estimée le16 avril 2025

Toutes les photos(1)

Sélectionner une taille de conditionnement

Changer de vue
50 UNITS
747,00 €

About This Item

Numéro CAS:
52227-83-5
Numéro MDL:
MFCD00166444
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

747,00 €

Date d'expédition estimée le16 avril 2025

Source biologique

bacterial (Flavobacterium heparinum)

Niveau de qualité

200

Conjugué

(Glucosaminoglycan)

Forme

lyophilized powder (with BSA as stabilizer)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Application

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.
The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide.[1] It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.[2]

Actions biochimiques/physiologiques

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.[3]

Définition de l'unité

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000282814
0000285693
0000294092
SLCQ4035
0000269784

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Zhenqing Zhang et al.
Analytical biochemistry, 385(1), 57-64 (2008-11-11)
Liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. Next
Yvette M Coulson-Thomas et al.
Journal of neuroscience methods, 171(1), 19-29 (2008-04-18)
Injury to the CNS of vertebrates leads to the formation of a glial scar and production of inhibitory molecules, including chondroitin sulphate proteoglycans. Various studies suggest that the sugar component of the proteoglycan is responsible for the inhibitory role of
Y M Michelacci et al.
The Biochemical journal, 151(1), 121-129 (1975-10-01)
A chondroitinase that degrades only chondroitin sulphate B was isolated from Flavobacterium heparinum, and separated from a constitutive chondroitinase AC also present in extracts of F. heparinum. The enzyme acts only on chondroitin sulphate B, producing oligo- and tetra-saccharides, plus
Nicola Volpi et al.
Glycobiology, 19(4), 356-367 (2008-12-06)
Glycosaminoglycans from the body of marine clam Scapharca inaequivalvis were extracted at about 0.15- 0.18 mg/g of dry tissue, composed of dermatan sulfate (DS) (approx. 74%) and heparan sulfate (26%). After treatment with nitrous acid, DS was isolated for further
Gerdy B ten Dam et al.
The American journal of pathology, 171(4), 1324-1333 (2007-08-25)
Chondroitin sulfate (CS) is abundantly present in the tumor stroma, and tumor-specific CS modifications might be potential targets to influence tumor development. We applied the phage display technology to select antibodies that identify these tumor-specific CS modifications. Antibody GD3G7 was
Afficher tous les articles scientifiques apparentés

Articles

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Questions

Évaluations

Aucune valeur de notation

Filtres actifs

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique