Accéder au contenu
Merck
Toutes les photos(1)

Documents

C5867

Sigma-Aldrich

Anti-Ciliated Cell Marker antibody, Mouse monoclonal

clone LhS 28, purified from hybridoma cell culture

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD08705577
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

LhS 28, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~45 kDa

Espèces réactives

human, hamster

Concentration

~2 mg/mL

Technique(s)

electron microscopy: suitable
immunocytochemistry: 1-2 μg/mL using BHK a21 cells
immunohistochemistry: suitable
microarray: suitable
western blot: suitable

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Anti-Ciliated Cell Marker antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma LhS 28 produced by the fusion of mouse myeloma cells (Sp2/0 cells) and splenocytes from BALB/c mice.

Spécificité

Anti-Ciliated Cell Marker antibody, Mouse monoclonal recognizes an antigen in human and hamster ciliated cells.

Immunogène

BHK a21 cytoskeletal preparation.

Application

Anti-Ciliated Cell Marker antibody, Mouse monoclonal may be used in:
  • immunoelectron microscopy
  • immunoblotting
  • immunohistochemistry
  • immunocytochemistry

May be used in the detection of ciliated cells and for investigating mechanisms of ciliogenesis.

Actions biochimiques/physiologiques

The mouse hybridoma LhS 28 secretes a monoclonal antibody that recognizes the antigen 44-45 kDa protein associated with the ciliary basal bodies. The antigen recognized by LhS 28 is present in the 9+0 microtubule arrays in the basal body structure of the cilium. It serves as an unequivocal marker for in situ detection and in vitro studies of human ciliated epithelial cells. LhS28 is a potential marker for tubal metaplasia identification and may be useful in recognizing low grade cervical glandular intraepithelial neoplasia. It is effective in detecting ciliogenesis and ciliated cells associated with normal and pathological human specimens

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M T Comer et al.
Journal of clinical pathology, 52(5), 355-357 (1999-11-24)
The assessment of neoplastic disease in gynaecological histopathology can be complicated by the high incidence of metaplasia seen in tissues of the female genital tract. There is a need to identify specific tissue markers which can be applied in routine
M T Comer et al.
The Histochemical journal, 31(1), 39-43 (1999-07-16)
The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M(r) protein specifically associated

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique