This product is provided as a solution with a minimum concentration of 0.50 mg protein/mL Carboxypeptidase B in 0.05 M NaOAc pH 5.0, containing 1.0 M NaCl and 0.01% NaN3. The concentration is lot-specific and reported in the product Certificate of Analysis. The maximum volume, based on the product specification, is 0.2 mL. This product is stored at −20°C and ships on dry ice. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/c5233#product-information
C5233
Carboxypeptidase B from human pancreas
50-55 units/mg protein carboxypeptidase B
Synonyme(s) :
Peptidyl-L-Lysine[L-arginine] hydrolase
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100 μG
1 650,00 €
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100 μG
1 650,00 €
About This Item
1 650,00 €
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Source biologique
human pancreas
Niveau de qualité
Forme
solution
Activité spécifique
50-55 units/mg protein carboxypeptidase B
Impuretés
≤0.2% chymotrypsin
≤0.2% trypsin
≤1 unit/mg protein carboxypeptidase A
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Chaîne SMILES
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
Clé InChI
TWURVFFNODFJBJ-UHFFFAOYSA-N
Informations sur le gène
human ... CPB1(1360)
Catégories apparentées
Description générale
Application
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat.[3] The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.[4]
Actions biochimiques/physiologiques
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro.[5] The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Définition de l'unité
One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C
Forme physique
Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Les clients ont également consulté
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The Journal of biological chemistry, 280(25), 23987-24003 (2005-04-23)
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The Biochemical journal, 239(3), 763-767 (1986-11-01)
The plasma complement protein C4 is encoded at two highly polymorphic loci, A and B, within the class-III region of the major histocompatibility complex. At least 34 different polymorphic variants of human C4 have been identified, including non-expressed or 'null'
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Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 9(5), 700-707 (2009-08-18)
The concentration of carboxypeptidase B activation peptide (CAPAP) is proposed to be a predictor of severe acute pancreatitis. The activated protein C (APC)-protein C inhibitor (PCI; APC-PCI) complex in plasma could be useful in detecting the hypercoagulative condition in severe
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I have a question about the Carboxypeptidase B (C-5233). Generally, how much volume of buffer solution (0.05M NaOAc pH 5.0+1.0M NaCl+0.01% NaN3) to add to make the stock? What is the storage temp. for the stock solution after reconstitution? Thank you!
1 réponse-
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