Cleaves the substrates consisting of consensus sequence DEVD.
Member of the CED-3 subfamily, the effector caspases, with an essential role in the apoptotic process. Upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions. It is responsible for the cleavage of cellular substrates that precipitate the morphological changes of apoptosis.
Member of the CED-3 subfamily, the effector caspases; upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions.
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses.
Définition de l'unité
One unit will hydrolyze 1.0 nmol of Ac-Asp-Glu-Val-Asp-AFC per minute at pH 7.5 at 25 °C.
Forme physique
Solution in HEPES, NaCl, DTT and sucrose, without carrier protein
Remarque sur l'analyse
Migrates as two major polypeptides (20 kDa and 10 kDa) in immunoblotting. Two minor polypeptides derived from the large subunit are also present.
The Journal of biological chemistry, 273(18), 10815-10818 (1998-06-06)
Caspases plays a key role in the execution phase of apoptosis. "Initiator" caspases, such as caspase-8, activate "effector" caspases, such as caspase-3 and -7, which subsequently cleave cellular substrates thereby precipitating the dramatic morphological changes of apoptosis. Following treatment of
Questions
Évaluations
★★★★★ Aucune valeur de notation
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
