C2409
Creatinase from microorganisms
lyophilized powder, ≥4 units/mg solid
Synonyme(s) :
Creatine Amidinohydrolase
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1000 units | Vérifier la disponibilité des articles du panier | 514,00 € |
A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-471-1
MDL number:
Specific activity:
≥4 units/mg solid
Biological source:
bacterial (Actinobacillus spp.)
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Laissez-nous vous aiderbiological source
bacterial (Actinobacillus spp.)
Quality Segment
form
lyophilized powder
specific activity
≥4 units/mg solid
mol wt
~100 kDa
storage temp.
−20°C
General description
Creatinase is a homodimer that catalyzes hydrolysis of creatine.[1] It consists of two monomer subunits and two defined domains; N and C terminal domains.[2] The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.[3]In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent.[4]
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾2M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu++, Hg++, Ag+
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)
Michaelis constant: 1.9 x 10‾2M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu++, Hg++, Ag+
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)
Application
Biochem/physiol Actions
Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.[7]
Physical form
Lyophilized powder containing sugars and EDTA as stabilizers
Other Notes
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.
1 of 1
Cet article | |||
|---|---|---|---|
| specific activity ≥4 units/mg solid | specific activity 100-300 units/mg protein | specific activity ≥50 units/mg protein | specific activity ≥300 U/mg, 25 °C, 750 U/mg, 37 °C |
| biological source bacterial (Actinobacillus spp.) | biological source - | biological source Escherichia coli | biological source rabbit skeletal muscle |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
| mol wt ~100 kDa | mol wt ~175 kDa | mol wt 110 kDa | mol wt - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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