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Merck
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Principaux documents

C1872

Sigma-Aldrich

Anti-CPSF2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-100-KD subunit, Anti-CPSF100, Anti-Cleavage and polyadenylation specificity factor 2

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About This Item

Code UNSPSC :
12352203

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Forme

buffered aqueous solution

Poids mol.

antigen ~100 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 2-5 μg using A20 cell lysates
western blot: 2-4 μg/mL using K562 cell lysates

Numéro d'accès UniProt

Q9P2I0

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... CPSF2(53981)
mouse ... Cpsf2(51786)

Immunogène

synthetic peptide corresponding to amino acids 718-732 of human CPSF2 conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-CPSF2 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 2-5μg using A20 cell lysates and western blotting at a concentration of 2-4μg/mL using K562 cell lysates.

Actions biochimiques/physiologiques

Cleavage and polyadenylation specificity factor subunit 2 is a protein encoded by the CPSF2 gene in humans. It is also referred to as CPSF100. CPSF and cleavage stimulation factor (CstF) play an important role during the formation of the 3′ ends of mRNA for 3′ cleavage of the transcript as well as for subsequent polyadenylation. CPSF and poly(A) polymerase (PAP) activity is controlled by Eg2-catalyzed phosphorylation during formation of cytoplasmic polyadenylation complex.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
039K4760

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A Jenny et al.
Molecular and cellular biology, 14(12), 8183-8190 (1994-12-01)
During the formation of the 3' ends of mRNA, the cleavage and polyadenylation specificity factor (CPSF) is required for 3' cleavage of the transcript as well as for subsequent polyadenylation. Using peptide sequences from a tryptic digest, we have cloned
W Schul et al.
The EMBO journal, 15(11), 2883-2892 (1996-06-03)
The cleavage stimulation factor (CstF), and the cleavage and polyadenylation specificity factor (CPSF) are necessary for 3'-terminal processing of polyadenylated mRNAs. To study the distribution of 3' cleavage factors in the nuclei of human T24 cells, monoclonal antibodies against the
R Hodgman et al.
Development (Cambridge, England), 128(14), 2815-2822 (2001-08-30)
In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB, a sequence-specific RNA binding

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