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A9810

Sigma-Aldrich

Amyloid β Protein Fragment 1-42

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0.1 MG
242,00 €

242,00 €

Date d'expédition estimée le14 avril 2025

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About This Item

Formule empirique (notation de Hill):
C203H311N55O60S
Numéro CAS:
107761-42-2
Poids moléculaire :
4514.04
Numéro MDL:
MFCD00163049
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

242,00 €

Date d'expédition estimée le14 avril 2025

Devis pour commande en gros

Essai

≥95% (HPLC)

Niveau de qualité

200

Forme

powder

Numéro d'accès UniProt

P05067

Température de stockage

−20°C

Chaîne SMILES 

S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(

InChI

1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112

Clé InChI

DZHSAHHDTRWUTF-SIQRNXPUSA-N

Informations sur le gène

human ... APP(351)

Amino Acid Sequence

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

Description générale

Amyloid β Protein is produced from amyloid-β precursor protein (APP). It consists of two C terminal variants, such as a long tailed Aβ 1-42 and a short tailed Aβ 1-40.[1] APP is located on human chromosome 21q21.3.[2]

Application

Amyloid β Protein Fragment 1-42 has been used:
  • for the preparation of Aβ 1-42 oligomer[3][4]
  • for western blot analysis[5]
  • for the interference test of immunomagnetic reduction (IMR) plasma Aβ42 assay[6]
  • to study the effects of resveratrol on Aβ 1-42-induced impairment of spatial learning, memory and synaptic plasticity[7]
  • to investigate the effect of Aβ in epithelial cell culture[1]

Actions biochimiques/physiologiques

Amyloid β Protein Fragment 1-42 (Aβ 1-42) has antioxidant and neuroprotective properties.[8] Accumulation of amyloid β Protein is associated with Alzheimer′s disease (AD) and Down Syndrome.[9] Aβ 1-42 regulates cholesterol transport[10] and may function as a transcription factor.[11] It may possess anti-inflammatory and antimicrobial properties.[12]
The predominant fragment of amyloid β-protein found in the brains of patients with Alzheimer′s disease and Down′s syndrome.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000388520
0000387190
0000368432
0000336675
0000331858

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Magnesium Ions Inhibit the Expression of Tumor Necrosis Factor alpha and the Activity of gamma-secretase in a beta-amyloid Protein-dependent Mechanism in APP/PS1 Transgenic Mice
Yu X, et al.
Frontiers in Molecular Neuroscience, 11 (2018)
beta-amyloid expression in age-related cataract lens epithelia and the effect of beta-amyloid on oxidative damage in human lens epithelial cells
Xu J, et al.
Molecular Vision, 23, 1015-1015 (2017)
The role of Abeta circRNA in Alzheimer? s disease: alternative mechanism of Abeta biogenesis from Abeta circRNA translation
Mo D, et al.
bioRxiv, 260968-260968 (2018)
Plasma Amyloid-beta(Abeta 42) Correlates with Cerebrospinal Fluid Abeta 42 in Alzheimer?s Disease
Teunissen CE, et al.
Journal of Alzheimer'S Disease, 1-7 (2018)
J T Jarrett et al.
Biochemistry, 32(18), 4693-4697 (1993-05-11)
Several variants of the beta amyloid protein, differing only at their carboxy terminus (beta 1-39, beta 1-40, beta 1-42, and beta 1-43), have been identified as the major components of the cerebral amyloid deposits which are characteristic of Alzheimer's disease.
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Questions

1–10 sur 10 questions  

  1. After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?

    1 réponse

    1. This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Utile ?

  2. What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?

    1 réponse

    1. These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
      PP69 Calbiochem:
      https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
      A9810 Sigma-Aldrich:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Utile ?

  3. It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution

    1 réponse

    1. This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.

      Utile ?

  4. How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?

    1 réponse

    1. It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.

      Utile ?

  5. in which solvent it should be dissolved ?

    1 réponse

    1. As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.

      See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Utile ?

  6. Is the mouse protein fragment the same as the human protein fragment in Product A9810, Amyloid β Protein Fragment 1-42?

    1 réponse

    1. The homology is 93% by identity, 95% positives...  no gaps

      Utile ?

  7. What is the source of Product A9810, Amyloid β Protein Fragment 1-42?

    1 réponse

    1. This protein fragment is a synthetic organic.  The gene for this peptide is located on human chromosome 21.

      Utile ?

  8. What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?

    1 réponse

    1. The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992

      Utile ?

  9. What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?

    1 réponse

    1. The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.

      Utile ?

  10. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile ?

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