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A9415
Actinomycin D
from Streptomyces sp., suitable for cell culture, ≥95%
Synonyme(s) :
Cosmegen, Meractinomycin, Actinomycin C1, Actinomycin IV, Dactinomycin
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About This Item
Formule empirique (notation de Hill):
C62H86N12O16
Numéro CAS:
Poids moléculaire :
1255.42
Beilstein:
605235
Numéro CE :
Numéro MDL:
Code UNSPSC :
51282001
ID de substance PubChem :
Nomenclature NACRES :
NA.76
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
Streptomyces sp.
Niveau de qualité
Essai
≥95%
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Couleur
red, powder
Pf
251-253
Solubilité
ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)
Spectre d'activité de l'antibiotique
neoplastics
Mode d’action
DNA synthesis | interferes
Température de stockage
2-8°C
Chaîne SMILES
CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O
InChI
1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
Clé InChI
RJURFGZVJUQBHK-IIXSONLDSA-N
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Catégories apparentées
Description générale
Chemical structure: peptide
Application
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells.. Recommended for us in cell culture applications at 1μg/mL.
Actions biochimiques/physiologiques
Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.
Attention
This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.
Notes préparatoires
Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.
Autres remarques
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 2 Oral - Carc. 1B - Repr. 1B
Code de la classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Certificats d'analyse (COA)
Lot/Batch Number
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Articles
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Protocoles
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
Protocole du test au WST-1 pour la mesure de la viabilité, de la prolifération, de l'activation et de la toxicité cellulaires. Instructions de préparation du réactif WST-1 et exemples d'applications. Foire aux questions et guide de résolution des problèmes liés au test au WST-1.
Le dosage colorimétrique au service de la quantification non radioactive de la prolifération, de la viabilité et de la toxicité cellulaires sur cellules adhérentes ou en suspension cultivées sur des microplaques de 96 puits.
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
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What is the Department of Transportation shipping information for this product?
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What is the working concentration for Actinomycin D (Product No. A9415)?
1 réponse-
For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes. Suggested working concentrations is 1 μg/mL.
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How do you recommend dissolving Product No. A9415 (Actinomycin D)?
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Stock solutions of this product may be prepared at 10 mg/mL in either ethanol (EtOH) or dimethyl sulfoxide (DMSO).
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How long are stock solutions of Actinomycin D (Product No. A9415) stable?
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Stock solutions of this product in DMSO or ethanol should be stored in foil-wrapped vials at -20 °C. In DMSO, solution can be stored for up to 1 month at -20 °C. Do not store in glass vials.
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