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A9207

Sigma-Aldrich

Avidin−Agarose from egg white

aqueous glycerol suspension

Synonyme(s) :

Avidin agarose

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About This Item

Numéro MDL:
MFCD00130572
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.56

Source biologique

egg white

Conjugué

agarose conjugate

Forme

aqueous glycerol suspension

Matrice

cross-linked 6% beaded agarose

Activation de la matrice

epichlorohydrin

Fixation de matrice

amino

Espaceur de matrice

15 atoms

Température de stockage

−20°C

Catégories apparentées

Description générale

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
  • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
  • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
  • in avidin bead assay for purification of labelled biotin
  • for capture of glycoproteins from murine cornea samples

Actions biochimiques/physiologiques

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Définition de l'unité

One unit will bind 1.0 μg of d-biotin.

Forme physique

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Systematic evaluation of avidin-biotin interaction by fluorescence spectrophotometry
Sun, S., et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 89C, 99-104 (2011)
Dielectric spectroscopy of molecular interactions based on the avidin-biotin complex
Mellor, B.L., et al.
Conference proceedings : Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 3660-3663 (2011)
Covalent modification of cytoskeletal proteins in neuronal cells by tryptamine-4, 5-dione
Kato Y, et al.
Redox Biology, 2, 983-990 (2014)
Amber L Jolly et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(6), 2393-2404 (2017-02-23)
Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
Hytonen VP, et al.
The Biochemical Journal, 384(2), 385-390 (2004)
Afficher tous les articles scientifiques apparentés

Contenu apparenté

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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