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A7434

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

Numéro MDL:
MFCD00162626
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous glycerol solution

Technique(s)

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

Spécificité

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

Immunogène

Purified mouse IgG, Fc fragment

Application

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.

Autres remarques

Antibody adsorbed with human IgG and rat serum proteins.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background staining with human or rat samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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