A6781
Acetate Kinase from Bacillus stearothermophilus
lyophilized powder, 400-1,200 units/mg solid
Synonyme(s) :
ATP:Acetate phosphotransferase, Acetate Kinase Bacillus stearothermophilus
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
32160410
Produits recommandés
Source biologique
bacterial (Bacillus stearothermophilus)
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
400-1,200 units/mg solid
Température de stockage
2-8°C
Application
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
Actions biochimiques/physiologiques
Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 % α-helix and 43 % unordered structure.
Involved in the metabolism of propanoate, pyruvate and taurine.
Définition de l'unité
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.
Forme physique
Contains Tris-HCl buffer.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Met. Corr. 1
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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H Nakajima et al.
Journal of biochemistry, 86(5), 1169-1177 (1979-11-01)
Fructose 1,6-bisphosphate (FBP) stimulates the reaction of Bacillus stearothermophilus acetate kinase (AK). FBP changes the reaction curve for ATP from a sigmoidal type to a Michaelis-Menten one. The binding of FBP to AK was studied by an equilibrium dialysis method
H Nakajima et al.
Journal of biochemistry, 84(1), 193-203 (1978-07-01)
1. Acetate kinase [EC 2.7.2.1] from an thermophile, B. stearothermophilus, was purified and crystalized. 2. This enzyme was shown to be a tetramer of identical subunits which had a molecular weight of about 40,000. Amino acid analysis showed no SH
Sagar Chittori et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 67(Pt 12), 1658-1661 (2011-12-06)
Acetate kinase (AckA) catalyzes the reversible transfer of a phosphate group from acetyl phosphate to ADP, generating acetate and ATP, and plays a central role in carbon metabolism. In the present work, the gene corresponding to AckA from Salmonella typhimurium
Kuo-Hsiang Tang et al.
The Journal of biological chemistry, 285(46), 35848-35854 (2010-07-24)
The anoxygenic green sulfur bacteria (GSBs) assimilate CO(2) autotrophically through the reductive (reverse) tricarboxylic acid (RTCA) cycle. Some organic carbon sources, such as acetate and pyruvate, can be assimilated during the phototrophic growth of the GSBs, in the presence of
V Molle et al.
Molecular microbiology, 36(6), 1265-1278 (2000-08-10)
whiK was one of five new whi loci identified in a recent screen of NTG-induced whi mutants and was defined by three mutants, R273, R318 and R655. R273 and R318 produce long, tightly coiled aerial hyphae with frequent septation. In
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