A6691
Amidase from Pseudomonas aeruginosa
recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)
Synonyme(s) :
Acrylamide Amidohydrolase, Acylase
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
250 units | Vérifier la disponibilité des articles du panier | 388,00 € |
1000 units | Vérifier la disponibilité des articles du panier | 745,00 € |
A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Recombinant:
expressed in E. coli
Concentration:
14 mg/mL
Service technique
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expressed in E. coli
Quality Segment
form
buffered aqueous glycerol solution
hydroxamate transferase activity
≥200 units/mg protein (biuret)
concentration
14 mg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
Pseudomonas aeruginosa PAO1 ... PA4163(880181)
Application
Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.[1]
The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater [2].
Biochem/physiol Actions
The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment [2].
Physical form
Solution in 50% glycerol containing 7 mM 2-mercaptoethanol and phosphate buffer salt
Other Notes
One unit will convert 1.0 μmole of acetamide and hydroxylamine to acetohydroxamate and ammonia per min at pH 7.2 at 37 °C.
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Cet article | |||
|---|---|---|---|
| Gene Information Pseudomonas aeruginosa PAO1 ... PA4163(880181) | Gene Information cow ... ADA(280712) | Gene Information - | Gene Information - |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form lyophilized powder | form suspension |
| concentration 14 mg/mL | concentration - | concentration - | concentration - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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