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A0481

Sigma-Aldrich

Anti-Axin1(C-terminal)

enhanced validation

1.5 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-Axin, Anti-Axis Inhibitor-1, Anti-Axis inhibition protein 1

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About This Item

Numéro MDL:
MFCD02095870
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Poids mol.

antigen ~130 kDa

Espèces réactives

rat, mouse

Conditionnement

antibody small pack of 25 μL

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

1.5 mg/mL

Technique(s)

western blot: 0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1.

Numéro d'accès UniProt

O35625

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... AXIN1(8312)
mouse ... Axin1(12005)
rat ... Axin1(79257)

Description générale

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC),,,.
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide

Immunogène

synthetic peptide corresponding to amino acids 815-832 located near the C-terminal of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in human, mouse, and bovine axin1.

Application

Anti-Axin1 (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1). The antibody has also been used for immunoblotting assays in astrocytoma cell lysates.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
126K4875

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Lobna Elkhadragy et al.
Frontiers in oncology, 12, 904031-904031 (2022-06-08)
Hepatocellular carcinoma (HCC) is an aggressive disease lacking effective treatment. Animal models of HCC are necessary for preclinical evaluation of the safety and efficacy of novel therapeutics. Large animal models of HCC allow testing image-guided locoregional therapies, which are widely
L Zeng et al.
Cell, 90(1), 181-192 (1997-07-11)
Mutations at the mouse Fused locus have pleiotropic developmental effects, including the formation of axial duplications in homozygous embryos. The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins. Mutant Fused alleles
F Fagotto et al.
The Journal of cell biology, 145(4), 741-756 (1999-05-20)
Axin was identified as a regulator of embryonic axis induction in vertebrates that inhibits the Wnt signal transduction pathway. Epistasis experiments in frog embryos indicated that Axin functioned downstream of glycogen synthase kinase 3beta (GSK3beta) and upstream of beta-catenin, and
Li-Ying Zhang et al.
International journal of oncology, 35(1), 25-32 (2009-06-11)
Astrocytic tumors are the most common brain tumors with various genetic defects. As a tumor suppressor gene, Axin could control cell death and growth. Axin possesses a separate domain that directly interacts with p53 and regulates the activity of p53
H Yamamoto et al.
Molecular and cellular biology, 18(5), 2867-2875 (1998-05-05)
Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3beta (GSK-3beta). This protein had 44% amino acid identity with Axin, a negative regulator of the Wnt signaling pathway. We designated this protein Axil
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