RNB300
GenElute™ Single Cell RNA Purification Kit
Synonyme(s) :
RNA extraction kit for NGS, RNA extraction kit for qPCR, RNA isolation kit for NGS, RNA isolation kit for qPCR, RNA purification kit for NGS, RNA purification kit for qPCR, single cell RNA extraction kit, single cell RNA isolation kit, single cell RNA kit, single cell total RNA extraction kit, single cell total RNA isolation kit, single cell total RNA purification kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41105600
Nomenclature NACRES :
NA.52
Produits recommandés
Caractéristiques du produit alternatif plus écologique
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Autre catégorie plus écologique
Conditions d'expédition
ambient
Température de stockage
room temp
Description générale
Sigma′s GenElute™ Single Cell RNA Purification Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). The kit can recover RNA from as little as a single cell to 2 x 10^5 cells. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
- Fast and easy processing using rapid micro spin-column format
- Small elution volume of 8 μL
- Isolate total RNA, from large rRNA down to microRNA (miRNA) without compromising total yield
- No phenol or chloroform extractions
- Isolate high quality total RNA from a variety of sources
- RNA can be isolated and detected from as little as a single animal cell
Application
- Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
- RT-PCR for both large mRNA and small RNA including miRNA
- Expression profiling
- Next Generation Sequencing for RNA and miRNA
- microRNA cloning and amplification
- Northern blotting
- RNase protection
- Primer extension
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
Caractéristiques et avantages
- Ideal for small inputs - Total RNA can be isolated from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). RNA has been isolated from as little as a single cell.
- Smal elution volume - Elute total RNA in as little as 8 μL of Elution Buffer
- No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
- Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) without compromising yield
- Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes
Définition de l'unité
Preps
Autres remarques
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Informations légales
GenElute is a trademark of Sigma-Aldrich Co. LLC
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Risques supp
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 2
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Shasha Zhang et al.
Cellular and molecular life sciences : CMLS, 77(7), 1401-1419 (2019-09-06)
Foxg1 is one of the forkhead box genes that are involved in morphogenesis, cell fate determination, and proliferation, and Foxg1 was previously reported to be required for morphogenesis of the mammalian inner ear. However, Foxg1 knock-out mice die at birth
Yuxian Guo et al.
Nature communications, 12(1), 7094-7094 (2021-12-09)
Oxidative stress contributes to the pathogenesis of acute lung injury. Protein S-glutathionylation plays an important role in cellular antioxidant defense. Here we report that the expression of deglutathionylation enzyme Grx1 is decreased in the lungs of acute lung injury mice.
Tao Gao et al.
JCI insight, 6(10) (2021-04-14)
Retinoic acid (RA) signaling is essential for enteric nervous system (ENS) development, since vitamin A deficiency or mutations in RA signaling profoundly reduce bowel colonization by ENS precursors. These RA effects could occur because of RA activity within the ENS
Dandan Liang et al.
Nature communications, 12(1), 287-287 (2021-01-14)
Bioelectrical impulses intrinsically generated within the sinoatrial node (SAN) trigger the contraction of the heart in mammals. Though discovered over a century ago, the molecular and cellular features of the SAN that underpin its critical function in the heart are
Shaoxue Zeng et al.
Theranostics, 12(15), 6705-6722 (2022-10-04)
Rationale: Müller cells play an essential role in maintaining the health of retinal photoreceptors. Dysfunction of stressed Müller cells often results in photoreceptor degeneration. However, how these cells communicate under stress and the signalling pathways involved remain unclear. In this
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
