66540200
WTSIi084-B
Human iPS Cell Line
Synonyme(s) :
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
About This Item
Source biologique
human dermis (fibroblast)
Niveau de qualité
Méthode de reprogrammation
Sendai virus
Description
age (N/A)
Fabricant/nom de marque
EBiSC™
Sexe
male
Mode de croissance
adherent (pluripotent)
Technique(s)
cell culture | stem cell: suitable
Maladie(s) pertinente(s)
none
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Origine de la lignée cellulaire
Wellcome Trust Sanger Institute
Description de la lignée cellulaire
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi084-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)NANOG(+)SOX2 (+)
PluriTest: Pluripotency score: 32.464
Novelty score: 1.359
Microarray data: https://www.ebi.ac.uk/ega/datasets/EGAD00010000775
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Liaison
Procédure de repiquage
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°C
Informations légales
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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