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Sigma-Aldrich

Phalloidin–Atto 647N

BioReagent, suitable for fluorescence, ≥80% (HPLC)

Synonyme(s) :

Atto 647N, Atto 647N-Phalloidin

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About This Item

Code UNSPSC :
12171501
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Pureté

≥80% (HPLC)

Fabricant/nom de marque

ATTO-TEC GmbH

λ

in methanol

Absorption UV

λ: 640-646 nm Amax

Adéquation

suitable for fluorescence

Méthode de détection

fluorometric

Température de stockage

−20°C

Catégories apparentées

Description générale

Phalloidin–Atto 647N is a new fluorescentlabel targeting the red spectral region. Atto 647N is a cationic dye, and postcoupling, it carries a net electrical charge of +1.Like most Atto labels, the absorption andfluorescence of Atto 647N are independent of pH between 2-11. Atto 647N issupplied in the form of a mixture containing two isomers having identicalfluorescence and absorption properties. Atto labels have rigid structures thatdo not show any cis-trans-isomerization.

Application

Phalloidin–Atto 647Nis designed to be used for labelling DNA, RNA, or proteins. Fluorescentconjugates of phalloidin are used to label actin filaments for histologicalapplications. Some structural features of phalloidin are required for thebinding to actin.

Caractéristiques et avantages

Characteristic features of the Phalloidin Atto488 are:
  1. StrongAbsorption.
  2. HighFluorescence quantum yield.
  3. HighPhotostability.
  4. MinimalTriplet formation.
  5. GoodSolubility.
  6. Excellent Ozone Resistance.

Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Pictogrammes

Skull and crossbones

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Catherine Pfefferli et al.
Nature communications, 8, 15151-15151 (2017-05-04)
The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which
Georgios Trichas et al.
BMC biology, 6, 40-40 (2008-09-17)
Transgenic animals are widely used in biomedical research and biotechnology. Multicistronic constructs, in which several proteins are encoded by a single messenger RNA, are commonly used in genetically engineered animals. This is currently done by using an internal ribosomal entry
Benedetta Artegiani et al.
Nature cell biology, 22(3), 321-331 (2020-03-04)
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. Here, we describe CRISPR-Cas9-mediated homology-independent organoid transgenesis (CRISPR-HOT), which enables efficient generation
Francesca Farina et al.
The EMBO journal, 38(11) (2019-04-25)
Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule
Laura F Corns et al.
Nature communications, 9(1), 4015-4015 (2018-10-03)
In the adult auditory organ, mechanoelectrical transducer (MET) channels are essential for transducing acoustic stimuli into electrical signals. In the absence of incoming sound, a fraction of the MET channels on top of the sensory hair cells are open, resulting

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