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Sigma-Aldrich

Anti-Rabbit IgG F(ab′)2 fragment - Atto 488, whole molecule antibody produced in goat

1 mg/mL protein

Synonyme(s) :

Atto 488-[F(ab′)2 fragment goat-Anti-rabbit IgG], whole molecule

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

Atto 488 conjugate

Forme d'anticorps

F(ab′)2 fragment of affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Contient

50% glycerol as stabilizer

Espèces réactives

rabbit

Concentration

1 mg/mL protein

Fluorescence

λex 500 nm; λem 522 nm in PBS

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs associate with rabbit IgGs.

Immunogène

rabbit IgG F(ab′)2 fragment

Application

Atto 488-goat anti-rabbit IgG F(ab′)2 fragment, whole molecule antibody can be used for fluorescence-based immunoassays.

Forme physique

Atto 488 F(ab)′2 fragment goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1mg/mL solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Notes préparatoires

F(ab)′2 fragment goat anti-rabbit is conjugated to Atto 488 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye.

Remarque sur l'analyse

Unconjugated dye ≤5% of total fluorescence; dye-to protein ratio ≥2

Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Janosch P Heller et al.
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