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04529

Nitrilase

recombinant, expressed in E. coli, ≥2.0 U/mg

Synonyme(s) :

Nitrile aminohydrolase

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A propos de cet article

Numéro CAS:
9024-90-2
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
3.5.5.1 (BRENDA, IUBMB)
MDL number:
MFCD04040969
Specific activity:
≥2.0 U/mg
Recombinant:
expressed in E. coli

recombinant

expressed in E. coli

form

crystals, powder

specific activity

≥2.0 U/mg

mol wt

41 kDa

storage temp.

2-8°C

General description

Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).

Application

Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.

Biochem/physiol Actions

Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid

Pictogrammes

Health hazard
GHS08

Mot-clé

Danger

Codes de danger

H317,H334

Mentions de précaution

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Classe de stockage

11 - Combustible Solids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Lot/Batch Number

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Questions

1–3 sur 3 questions  

  1. what is the bacteria or organism whose genome sequence have been expressed in E.Coli for nitrilase procurement? I need the name of the organism for research purpose

    1 réponse

    1. This enzyme is a recombinant protein produced in e. coli. The biological source and sequence of the protein is considered proprietary and may not be shared.

      Utile ?

  2. Can we get the source of the bacteria or organism whose genome sequence is being used for recombinant expressing in E.Coli for procuring nitrilase. Used for research purspose by a student

    1 réponse

    1. Unfortunately, the source and sequence is considered proprietary.

      Utile ?

  3. Is the nitrilase been synthesized through DNA recombinant technology or is there any other source organism from which the nitrilase has been procured?

    1 réponse

    1. Yes, this product is a recombinant protein expressed in E. coli.

      Utile ?

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