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Principaux documents

H2899

Supelco

HPLC protein standard mixture

analytical standard

Synonyme(s) :

Protein Calibration Mix

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1 VIAL
221,00 €

221,00 €

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1 VIAL
221,00 €

About This Item

Code UNSPSC :
85151701
Nomenclature NACRES :
NA.24

221,00 €

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Devis pour commande en gros

Qualité

analytical standard

Niveau de qualité

200

Forme

solid

Classe(s) chimique(s) de l'analyte

amino acids, peptides, proteins

Technique(s)

HPLC: suitable

Application(s)

food and beverages

Format

multi-component solution

Température de stockage

−20°C

Catégories apparentées

Application

HPLC protein standard mixture has been used as a standard for the separation of proteins in cell extract samples of bacteria using high performance liquid chromatography (HPLC).[1]

Conditionnement

Vial contains approximately 1 mg each of ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000333965
0000333965
SLCQ6184
SLCK5754
SLCF7915

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Distribution, diversity, and activities of sulfur dioxygenases in heterotrophic bacteria
Liu H, et al.
Applied and Environmental Microbiology, 80, 1799-1806 (2014)
Honglei Liu et al.
Applied and environmental microbiology, 80(5), 1799-1806 (2014-01-07)
Sulfur oxidation by chemolithotrophic bacteria is well known; however, sulfur oxidation by heterotrophic bacteria is often ignored. Sulfur dioxygenases (SDOs) (EC 1.13.11.18) were originally found in the cell extracts of some chemolithotrophic bacteria as glutathione (GSH)-dependent sulfur dioxygenases. GSH spontaneously

Questions

1–4 sur 4 questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Could you provide an order of retention times of when each of these components elute off a protein C4 column (H2899 protein mixture)?  Thank you.

    1 réponse

    1. The exact method is considered proprietary. However, for a C4 column, it should elute ribonuclease A first, followed by cytochrome C, holo-transferrin, and apomyoglobin.

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  4. What is each componant's actual molecular weight (ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin)?

    1 réponse

    1. The molecular weight of each component is not reported. See below for the expected values:
      Ribonuclease A: approx. 13.7 kDa
      Cytochrome C: approx. 12.3 kDa
      holo-Transferrin: 72.1 - 78.5 kDa
      Myoglobin: approx. 17.6 kDa

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