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61768

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Lectin, immobilized on Agarose CL-4B from Triticum vulgaris

suitable for affinity chromatography

Synonyme(s) :

Wheat germ agglutinin, immobilized on agarose CL-4B

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About This Item

Code UNSPSC :
12190000
Nomenclature NACRES :
NA.21

Technique(s)

affinity chromatography: suitable

Niveau de qualité

Capacité

0.1-1.0 mg/mL, packed gel capacity (ovomucoid)

Température de stockage

2-8°C

Forme physique

50% aqueous suspension in 1 M NaCl

Autres remarques

Protein isolation from bordetella pertussis that facilitates entry of calmodulin-sensitive adenylate cyclase into animal cells

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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M G Donovan et al.
Biochemistry, 28(20), 8124-8129 (1989-10-03)
Bordetella pertussis, the pathogen responsible for whooping cough, releases a soluble calmodulin-sensitive adenylate cyclase into its culture medium. Several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular cAMP levels [Confer

Articles

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This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

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