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Merck
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48305

Millipore

Bile salts

suitable for microbiology

Synonyme(s) :

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture, Bile acids sodium salt, Cholic acid-Deoxycholic acid sodium salt mixture

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About This Item

Numéro MDL:
MFCD00130648
Code UNSPSC :
41106212
Nomenclature NACRES :
NA.85

Description

anionic

Niveau de qualité

200

Stérilité

non-sterile

Pureté

40-60% (Cholic Acid ; approximately 50% sodium cholate and approximately 50% sodium deoxycholate, HPLC)

Forme

powder

Composition

cholic acid sodium salt, ~50%
deoxycholic acid sodium salt, ~50%

Impuretés

≤5% water

Perte

≤5% loss on drying (water)

Solubilité

H2O: 0.1 g/mL, clear, faintly yellow

Application(s)

microbiology

Température de stockage

10-30°C

Catégories apparentées

Description générale

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.
Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.

Application

Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.

Actions biochimiques/physiologiques

Bile salts are surface-active steroid compounds and their primary physiological function is to aid in the digestion of lipophilic nutrients in intestinal tracts of vertebrates.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCK4542
BCCH7185
BCCJ5649
BCCJ5529
BCCG0220

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Bacterial degradation of bile salts.
Philipp B
Applied Microbiology and Biotechnology, 89 (4), 903-915 (2011)
Bonggyu Min et al.
Frontiers in bioengineering and biotechnology, 8, 828-828 (2020-08-15)
Stress resistance is an important trait expected of lactic acid bacteria used in food manufacturing. Among the various sources of stress, high temperature is a key factor that interrupts bacterial growth. In this regards, constant efforts are made for the
Eunsil Choi et al.
Frontiers in microbiology, 11, 597515-597515 (2020-11-27)
The BipA (BPI-inducible protein A) protein is ubiquitously conserved in various bacterial species and belongs to the translational GTPase family. Interestingly, the function of Escherichia coli BipA is not essential for cell growth under normal growth conditions. However, cultivation of
Luis Noriega et al.
International journal of food microbiology, 94(1), 79-86 (2004-06-03)
Six derivatives with increased resistance to ox gall (MIC: > or = 1% w/v) and one derivative resistant to sodium cholate (MIC: 0.8% w/v) were obtained from more sensitive original Bifidobacterium strains. These microorganisms, and two additional cholate resistant derivatives
Francesco Fancello et al.
Microorganisms, 8(1) (2020-01-16)
Bacterial diversity of 15 extra virgin olive oils, obtained from different Italian varieties, including Frantoio, Coratina, Bosana, and Semidana, was analyzed in this study. All bacterial isolates were genotyped using RAPD and REP-PCR method and grouped by means of cluster
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Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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