436143

Dodécylsulfate de sodium

ACS reagent, ≥99.0%

• Synonyme(s) : Dodécyl sulfate sodium salt, Dodécylsulfate de sodium, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Formule linéaire : CH₃(CH₂)₁₁OSO₃Na
• Numéro CAS: 151-21-3
• Poids moléculaire : 288.38
• Beilstein: 3599286
• Numéro CE : 205-788-1
• Numéro MDL: MFCD00036175
• Code UNSPSC : 12161900
• eCl@ss : 39093306
• ID de substance PubChem : 24867284
• Nomenclature NACRES : NA.21

Propriétés

• Qualité: ACS reagent
• Niveau de qualité: 200
• Description: anionic; 62
• Essai: ≥99.0%
• Forme: solid
• Poids mol.: 288.38 g/mol
• Impuretés: ≤0.06 meq/g Titr. Base; ≥96.0% fatty alcohols; <4.0% unsulfated alcohols
• Perte: ≤1.0% loss on drying
• Pf: 204-207 °C (lit.)
• Solubilité: H₂O: soluble 1 gm in 10 ml (opalescent solution)
• Traces de cations: heavy metals (as Pb): ≤0.002%
• λ: 3 %H₂O reference
• Absorption UV: λ: 220-350 nm A_max: ≤0.1
• HLB: 40
• Chaîne SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• Clé InChI: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

Description générale

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is a versatile anionic surfactant with a broad range of applications in cell biology, biochemistry, and various industries. Its amphiphilic nature, characterized by a hydrophobic hydrocarbon tail and a hydrophilic sulfate head group, makes it an effective detergent capable of solubilizing and denaturing proteins, lipids, and other hydrophobic molecules.

In protein biochemistry, SDS plays a pivotal role in protein extraction and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its ability to denature proteins by disrupting their secondary and tertiary structures allows for their uniform migration through a polyacrylamide gel matrix based on their molecular weight. This technique, SDS-PAGE, has become an indispensable tool for analyzing protein size and purity.

Beyond protein analysis, SDS also finds applications in nucleic acid extraction and hybridization techniques. Its detergent properties aid in the lysis of cells and the solubilization of cellular components, facilitating the extraction of DNA and RNA. This makes SDS a valuable reagent in various molecular biology protocols, including PCR (polymerase chain reaction) and Southern blotting. Sodium dodecyl sulfate stands as a versatile and widely used surfactant with a diverse range of applications due to its ability to solubilize, denature, and separate biological molecules for cell biology and biochemical research.

Application

Détergent anionique

Sodium dodecyl sulfate was used as a test compound for studying that whether Bacillus amyloliquefaciens KPS46 can induce priming, signaling, protein production as well as systemic protection in soybean (Glycine max L.) against several diseases.[1]

Actions biochimiques/physiologiques

Sodium dodecyl sulfate (SDS) is an anionic detergent that can form complexes with protein through hydrophobic interactions. Studies have reported that the hydrodynamic functions of protein-SDS complexes are governed by the length of their polypeptide chains. Thus, SDS-based electrophoretic techniques can separate protein molecules based on their molecular weights.[2] Additionally, SDS can solubilize cell membranes and can extract membrane-bound proteins.[3]

Caractéristiques et avantages

• Highly versatile surfactant for your molecular biology and biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Autres remarques

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Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H228,H302 + H332,H315,H318,H335,H412
• Conseils de prudence: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 4.1B - Flammable solid hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 338.0 °F
• Point d'éclair (°C): 170 °C
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges