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198099

Sigma-Aldrich

Methyl violet 2B

certified by the Biological Stain Commission

Synonyme(s) :

Basic Violet 1

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About This Item

Numéro CAS:
8004-87-3
Numéro C.I. (Colour Index):
42535
Numéro Beilstein :
8464808
Numéro MDL:
MFCD00012568
Code UNSPSC :
12171500
ID de substance PubChem :
24851861
Nomenclature NACRES :
NA.47

Qualité

certified by the Biological Stain Commission

Niveau de qualité

100

Forme

crystalline powder

Composition

Dye content, 75%

Pf

137 °C (dec.) (lit.)

λmax

584 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

Cl.C\N=C1/C=C\C(C=C1)=C(/c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C24H27N3.ClH/c1-25-21-12-6-18(7-13-21)24(19-8-14-22(15-9-19)26(2)3)20-10-16-23(17-11-20)27(4)5;/h6-17H,1-5H3;1H

Clé InChI

JFTBTTPUYRGXDG-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Methyl Violet 2B is a mixture of polymethylated (4,5 and 6) pararosaniline chlorides

Application

Methyl violet 2B has been used to stain human lung fibroblast cells (A549) in in vitro antiviral assay.

Actions biochimiques/physiologiques

Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes. Hence, the dye removal is of great importance.

Adéquation

Certified for use in Flemming triple stain with iodine for chromosomes; Newton′s crystal violet-iodine technique for chromatin and nucleoli; Lieb′s amyloid stain.

Mention d'avertissement

Danger

Mentions de danger

H302,H318,H351,H410

Classification des risques

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
SHBR1833
MKBD5246V
MKBG5819V
MKBF7373V
MKBD5246

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Annabelle Herrington-Symes et al.
Journal of drug targeting, 25(9-10), 881-890 (2017-08-19)
Increasingly complex proteins can be made by a recombinant chemical approach where proteins that can be made easily can be combined by site-specific chemical conjugation to form multifunctional or more active protein therapeutics. Protein dimers may display increased avidity for
Equilibrium and kinetic studies of methyl violet sorption by agricultural waste
Hameed B H
Journal of Hazardous Materials, 154(1-3), 204-212 (2008)
Methyl Violet: a Selective Agent for Differentiation of
Klebsiella pneumoniae from Enterobacter aerogenes and Other
Gram-Negative Organisms
CAMPBELL L M and ROTH I L
Applied Microbiology, 30, 258-261 (1975)
Expression of soluble and active interferon
consensus in SUMO fusion expression system
in E.coli
Peciak K
Protein Expression and Purification, 99 (2014), 18- 26 (2014)
Removal of cationic dye methyl violet 2B from water by cation exchange membranes
ShiouWu J
Journal of Membrane Science, 309(1-2), 239-245 (2008)
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