00879

8-Acetoxypyrene-1,3,6-trisulfonic acid trisodium salt

for fluorescence, ≥98.0% (TLC)

• Synonyme(s) : 1-Acetoxypyrene-3,6,8-trisulfonic acid trisodium salt, Trisodium-8-acetoxypyrene-1,3,6-trisulfonate
• Formule empirique (notation de Hill): C₁₈H₉Na₃O₁₁S₃
• Numéro CAS: 115787-83-2
• Poids moléculaire : 566.42
• Beilstein: 9538267
• Numéro MDL: MFCD00135685
• Code UNSPSC : 12352200
• ID de substance PubChem : 24845099

Propriétés

• Qualité: for fluorescence
• Essai: ≥98.0% (TLC)
• Pf: 238-248 °C (dec.) (lit.)
• Solubilité: DMF: soluble; DMSO: soluble; H₂O: soluble; methanol: soluble
• Fluorescence: λ_ex 460 nm; λ_em 510 nm in 0.1 M potassium phosphate pH 8.0 (esterase)
• Température de stockage: −20°C
• Chaîne SMILES: [Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

Description

Application

A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).

suitable as fluorogenic substrate for esterases

Autres remarques

Highly water soluble enzyme substrate for the fluorimetric assay of esterases at longwave excitation and emission wavelengths[1]