GPT-RO

Glutamate-Pyruvate Transaminase (GPT)

from pig heart

• Synonyme(s) : alanine aminotransferase
• Numéro de classification (Commission des enzymes): 2.6.1.2 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: pig heart
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: ≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
• Conditionnement: pkg of 1 mL (10105589001 [10 mg]); pkg of 2.5 mL (10737127001 [25 mg])
• Fabricant/nom de marque: Roche
• pH optimal: ~8.0
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.[1]

Application

Synthesis of unnatural L-amino acids from α-keto acids.

Qualité

Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT

Définition de l'unité

Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Notes préparatoires

Activator: Pyridoxalphosphate
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available