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11271164001

Roche

Interleukin-2, mouse (mIL-2)

recombinant (E. coli)

Synonyme(s) :

mIL-2, mouse interleukin-2

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About This Item

Code UNSPSC :
12352207

Source biologique

mouse

Niveau de qualité

100

Produit recombinant

expressed in E. coli

Pureté

>95% (SDS-PAGE)

Forme

solution

Puissance

<0.5 ng/mL EC50

Poids mol.

17,200 Da

Conditionnement

pkg of 10,000 U (5 μg, 1 ml)

Fabricant/nom de marque

Roche

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

inhibition assay: suitable

Impuretés

<0.1 EU/μgtested (LAL test)

Numéro d'accès UniProt

P04351

Température de stockage

−20°C

Informations sur le gène

mouse ... Il2(16183)

Catégories apparentées

Description générale

Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).
Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.

Spécificité

Mouse IL-2 is effective on mouse cells, but not on human cells.

Application

Recombinant murine IL-2 is produced in E. coli and exhibits the following:
  • Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
  • Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
  • Weakly inhibits the binding to human responder cells.
  • Shares identical biological and immunological activities with human IL-2.
  • Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
  • used for the stimulation of murine T-cells

Qualité

Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Séquence

Chain Length 149 AA
One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.

Définition de l'unité

EC50 definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).

Notes préparatoires

Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.

Autres remarques

Specific activity/EC 50: >2 x 106 U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).
For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

James Lee et al.
Methods in molecular biology (Clifton, N.J.), 506, 83-96 (2008-12-27)
In comparison to human T cells, efficient retroviral gene transfer and subsequent expansion of murine primary T cells is more difficult to achieve. Herein, we describe an optimized gene transfer protocol utilizing an ecotropic viral vector to transduce primary murine
Bergren W Crute et al.
Frontiers in immunology, 11, 592329-592329 (2020-11-17)
Among the areas of most impactful recent progress in immunology is the discovery of inhibitory receptors and the subsequent translation of this knowledge to the clinic. Although the original and canonical member of this family is FcγRIIB, more recent studies
Massimo Martinelli et al.
iScience, 26(1), 105860-105860 (2023-01-13)
The RNA-binding protein Pcbp2 is widely expressed in the innate and adaptive immune systems and is essential for mouse development. To determine whether Pcbp2 is required for CD4+ T cell development and function, we derived mice with conditional Pcbp2 deletion in
Anastasiia Kalinina et al.
STAR protocols, 2(1), 100368-100368 (2021-03-23)
Adoptive transfer therapy has great potential to treat diseases such as cancer as well as autoimmune and infectious diseases. Identification of chain-centric T cell receptors (TCRs) with the dominant-active antigen-specific α-chains (TCRα) can significantly improve the efficacy of adoptive cell therapy
Tae Joon Won et al.
Cellular signalling, 27(6), 1246-1252 (2015-03-13)
Small ubiquitin-like modifier (SUMO) 2 is a small protein that controls the activity and stability of other proteins by SUMOylation. In this study, T cell-specific SUMO2 overexpressing transgenic mice were generated to study the effect of SUMO2 on T lymphocytes.
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