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11204530001

Roche

ABTS™ Buffer

solution, pkg of 125 mL

Synonyme(s) :

buffer

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About This Item

Code UNSPSC :
12352204
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

solution

Niveau de qualité

100

Conditionnement

pkg of 125 mL

Fabricant/nom de marque

Roche

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.[1]

Application

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.[2][3][4] Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.

Notes préparatoires

Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

ABTS is a trademark of Roche

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

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Certificats d'analyse (COA)

Lot/Batch Number
50683600
44035300
38716400
32524400
75533000

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C Lindenthal et al.
Infection and immunity, 69(1), 52-57 (2000-12-19)
Enterotoxigenic Escherichia coli (ETEC) is capable of invading epithelial cell lines derived from the human ileum and colon. Two separate invasion loci (tia and tib) that direct noninvasive E. coli strains to adhere to and invade cultured human intestine epithelial
Bruce S Sachais et al.
Blood, 120(5), 1137-1142 (2012-05-12)
Rapid laboratory assessment of heparin-induced thrombocytopenia (HIT) is important for disease recognition and management. The utility of contemporary immunoassays to detect antiplatelet factor 4 (PF4)/heparin antibodies is hindered by detection of antibodies unassociated with disease. To begin to distinguish properties
Patrik Erlmann et al.
Molecular biology of the cell, 20(20), 4400-4411 (2009-08-28)
Deleted in Liver Cancer 1 (DLC1) is a GTPase-activating protein (GAP) with specificity for RhoA, RhoB, and RhoC that is frequently deleted in various tumor types. By inactivating these small GTPases, DLC1 controls actin cytoskeletal remodeling and biological processes such
H Matsuda et al.
The Japanese journal of experimental medicine, 54(3), 131-138 (1984-06-01)
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for

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