The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP.
Synonyme(s) :
Mouse ES Cell Media
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ESGRO Complete™ Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF and BMP, and during routine washing steps when passaging cells.
Application
Routine washing of cells.
Suitable for serum free culture of ES cells when used with LIF and BMP.
Suitable for use as a base medium for differentiation upon addition of differentiation factors of choice.
Medium suitable for use on feeder independent cell lines.
Forme physique
Basal serum free medium formulation without BMP or LIF.
pH 7.1-7.3
Stockage et stabilité
Store at -20°C. Refer to lot expiration date on label. After thawing, store at 4°C for one month. DO NOT REFREEZE.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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The cytokine leukemia inhibitory factor (LIF) drives self-renewal of mouse embryonic stem (ES) cells by activating the transcription factor STAT3. In serum-free cultures, however, LIF is insufficient to block neural differentiation and maintain pluripotency. Here, we report that bone morphogenetic
A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: where do you put your gates?
Hughes O. R. et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology null
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