SCT275

BioTracker^™ Green Late-Phase Autophagy Live Cell Probe

Human

• Synonyme(s) : autolysosome live cell probe, green autophagy probe
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.47

Propriétés

• product name: BioTracker^™ Green Late-Phase Autophagy Live Cell Probe,
• Source biologique: human
• Niveau de qualité: 100
• Conditionnement: vial of 20 nmol
• Fabricant/nom de marque: Millipore
• Technique(s): cell culture | stem cell: suitable; flow cytometry: suitable
• Conditions d'expédition: 2-8°C
• Température de stockage: 2-8°C

Description

Description générale

SCT275 is used to detect late phase autophagy in live cells. This small hydrophobic probe molecule is plasma membrane-permeable and incorporates in to the autophagosome. Following lysosome-autophagosome fusion, increased acidity in the autolysosome enhances fluorescence signal of the BioTracker^™ Green Late-Phase Autophagy Live Cell Probe.SPECTRAL CHARACTERISTICS: Excitation λ: 405 nmEmission λ: 525 nmRecommended laser/filter:Ex 350 - 450 nmEm 500 nm - 560 nm

Caractéristiques et avantages

Fluorescence microscopyFlow cytometry

Description de la cible

Autophagy is an intracellular degradation process, where dysfunctional proteins and organelles are degraded by a system of organelles. Aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome.

Stockage et stabilité

Store at -20 °C, protected from light.

Autres remarques

SCT275 is a small hydrophobic molecule live cell probe that localizes to the autolysosome. The probe fluoresces green as the autolysosome environment acidifies, enabling the assessment of autophagy by fluorescence microscopy and flow cytometry.Add 20 uL DMSO to tube of SCT275 and pipet to dissolve to make a 1 mM stock solution. Dilute with culture medium to 0.1 - 1.0 uM to make working solution.

Informations légales

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable