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SCM112

Sigma-Aldrich

3dGRO® Wnt3a Conditioned Media Supplement

Synonyme(s) :

Wnt3a Conditioned Media;Wnt3a CM;Wnt Conditioned Media

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.76

Conditionnement

1 ea of 50 mL

Fabricant/nom de marque

Millipore

Technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description de la lignée cellulaire

3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO® Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.SourceThe 3dGRO® Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.

Application

  • Appearance (color): clear, red liquid
  • Endotoxin: 2 EU/mL
  • Sterility Tested: No Growth/Pass
  • Mycoplasma: Negative
  • Media tested for function in supporting growth of human pancreatic organoids
  • Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells

Caractéristiques et avantages

Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.

Stockage et stabilité

Wnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.

Informations légales

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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